Spectrophotometric determination of 3-hydroxy-3-methylglutaryl coenzyme-A reductase inhibitors in pharmaceutical preparations

Abstract

Simple and accurate spectrophotometric methods are presented for the determination of five 3-hydroxy-3- methylglutaryl coenzyme-A (HMG-CoA) reductase inhibitors (statins), namely atorvastatin, fluvastatin, pitavastatin, rosuvastatin, and simvastatin, in pharmaceutical preparations. The methods are based on the reaction of drugs as n-electron donor with 7,7,8,8- tetracyanoquinodimethane as p-acceptors to give highly colored complex species. All variables were studied in order to optimize the reaction conditions. Beer's law was obeyed in the concentration ranges 4-20 μg mL-1 , 4-12 μg mL-1 , 0.8-2.4 μg mL-1 , 4-14 μg mL-1 , and 2.5-20 μg mL-1 for atorvastatin, fluvastatin, pitavastatin, rosuvastatin, and simvastatin, respectively. The proposed methods were successfully applied to the pharmaceutical preparations without any interference from excipients. © TÜBITAK.