Antibiotic Sensitivity and Evaluation of Plasmid Profile of Major Foodborne Pathogens

Abstract

This study revealed the reason behind the antibiotics resistance of isolated food-borne pathogens through their susceptibility testing to various antibiotics of choice. The results of the study revealed that resistance of the bacteria isolates which are Streptococcus sp., Staphylococcus aureus, Proteus vulgaris, Shigella sp., Escherichia coli, Pseudomonas aeruginosa, Bacillus sp. to different antibiotics varies and differs considerably. For instance, Pseudomonas aeruginosa showed resistance to Amoxicillin, Augmentin, Gentamicin and Tetracycline. Staphylococcus sp. isolated showed multiple resistances to Cloxacillin, Erythromycin, Amoxicillin, Augmentin and Gentamicin. Proteus vulgaris showed multiple resistances to five antibiotics in-vitro which are Augmentin, Nitrofurantoin, Amoxicillin, Cotrimoxazole and Nalidixic. The seven isolates were then assayed for plasmid profiling by agarose gel electrophoresis. All the isolates has plasmid with varying sizes of between 9–21kb. Further conjugative study will reveal more reason behind the resistance.