Quantitative inheritance of volatile pheromones and darcin and their interaction in olfactory preferences of female mice

Abstract

In this study, we examined how urine-borne volatile compounds (UVCs) and darcin of male mice are inherited from parents and interact to modulate the olfactory preferences of females using two inbred strains of mice, C57Bl/6 (C57) and BALB/c (BALB), and their reciprocal hybrids (BC = BALB♀ × C57♂; CB = C57♀ × BALB♂;). Chemical analysis revealed that the UVCs of C57BL/6 males were quantitatively distinguishable from those of BALB/c males. Darcin was detected in C57 urine, but not in BALB urine. The levels of UVCs and darcin in both BC and CB were intermediate between those of C57 and BALB. Behaviourally, C57 females consistently preferred BALB male urine over C57 or CB males despite that there are trace amounts of darcin in BALB urine. However, the preference for BALB urine disappeared in contact two-choice tests of BALB vs. BC pairs, and restored when recombinant darcin was added to BALB male urine. Our results suggested that both UVCs and darcin in male mice are quantitatively inherited and interact to affect the olfactory preferences of females.