'Active' one carbon generation in Saccharomyces cerevisiae

Abstract

A new mutation introducing a one carbon requirement (e.g., formate) for the glycine supplemented growth of a serine glycine auxotroph (ser1) was correlated with a lack of glycine decarboxylase activity. The presence of oxalate decarboxylase activity or glyoxylate decarboxylase activity did not overcome the one carbon requirement. Another mutation characterized by the absence of oxalate decarboxylase activity did not introduce a one carbon requirement. Another mutation characterized by the absence of oxalate decarboxylase activity did not introduce a one carbon requirement. The presence and physiological significance of glycine decarboxylase activity in Saccharomyces are thus inferred.