Antibacterial effect of protein and protein hydrolysates isolated from bacteria enterobacter hormaechei associated with marine algae sargassum sp.

Abstract

This research study is about the antibacterial potential of protein and its hydrolysates isolated from bacteria Enterobacter hormaechei associated with marine algae. The protein was isolated using a fractionation method with ammonium sulphate. The fractions were pre-purified through dialysis with buffer Tris HCl. Protein hydrolysates were obtained through hydrolysis using the pepsin enzyme. Antibacterial activity was conducted using Escherichia coli and Staphylococcus aureus. The inhibition zone was observed after incubation for 24 h, 48 h, and 72 h at 37 °C. Results show that F1 fraction of protein hydrolysates had the highest activity against Escherichia coli and Staphylococcus aureus at 72 h with inhibition zones of 16.30±0.03 mm and 16.70±0.03 mm, respectively. Protein hydrolysates had higher inhibitory activity than the protein fraction, so it provides an information base for further study on the antibacterial drugs from bacteria associated with marine algae.