Abstract
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in Cre- or FLP-mediated genomic manipulations.
Cite
CITATION STYLE
Buchholz, F., Angrand, P. O., & Stewart, A. F. (1996). A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs. Nucleic Acids Research, 24(15), 3118–3119. https://doi.org/10.1093/nar/24.15.3118
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