In Vitro Inhibition of Binding of Tumor Necrosis Factor (TNF)-α by Monoclonal Antibody to TNF Receptor on Glioma Cell and Monocyte

Abstract

The use of monoclonal antibodies to the tumor necrosis factor (TNF) receptors, the TNF-p55 receptor (TNF-p55R) and the TNF-p75 receptor (TNF-p75R), was evaluated to reduce the effects of TNF caused by binding to TNF-p75R. Competitive binding of anti-TNF-p55R (mAbp55R) and anti-TNF-p75R monoclonal antibodies (mAbp75R) with iodine-125-labeled TNF-a to GL-9 glioma cells and U937 histiocytic lymphoma cells was evaluated. The effects of mAbp55R and mAbp75R on the growth suppression by TNF-a of GL-9 cells and TNF-a production in U937 cells were also examined. mAbp75R bound to U937 cells competitively with TNF-a and suppressed TNF-a production by U937, but had no effect on the growth inhibition of GL-9 human glioma cell by TNF-a in vitro. These findings suggest that co-administration of TNF-p75R antagonist with TNF-a may decrease the toxicity of TNF-a administration resulting in a better therapeutic result. © 1998, The Japan Neurosurgical Society. All rights reserved.