Analytical and clinical performance of a homogeneous enzymatic LDL- cholesterol assay compared with the ultracentrifugation-dextran sulfate- Mg2+ method

Abstract

LDL-cholesterol (LDL-C) concentration is currently determined in most clinical laboratories by the Friedewald calculation. This approach has several limitations and may not meet the current total error requirement in LDL-C measurement of ≤ 12%. We evaluated the analytical and clinical performance of the direct N-geneous LDL-C assay (Equal Diagnostics). The N- geneous method correlated highly with the modified beta-quantification assay (r = 0.95; y = 0.91x + 70.6 mg/L; n = 199), and showed no significant effect of increased triglyceride or other common interferants and performed adequately in serum samples from nonfasting individuals. This assay demonstrated a mean total error of 6.7% over a wide range of LDL-C concentrations. In addition, at the medical decision cutoff points, this LDL- C assay showed positive predictive values of 78-95% and negative predictive values of 84-99%. We conclude that the N-geneous LDL-C meets the currently established analytical performance goals and appears to have a role in the diagnosis and management of hypercholesterolemic patients.