Development of highly selective and sensitive fluorescent and chemiluminescent reagents

Abstract

Fluorescence (FL), chemiluminescence (CL) and bioluminescence (BL) have been extensively focused on luminescence assays. Various FL and CL compounds have been synthesized and evaluated their FL and CL properties. This review describes development of highly selective and sensitive FL and CL reagents by derivatizing luminol, indole and polyphenol. Luminol derivatives were used for measuring enzyme activities and ultraviolet ray power. Indole derivatives with large stokes shift and long-lasting CL properties were developed. Compound which had enhancement effect on polyphenol CL was found. Highly sensitive CL polyphenol dendrimers were synthesized. © 2008 The Pharmaceutical Society of Japan.