Amperometric biosensor for cholesterol based on novel nanocomposite array gold nanoparticles/acetone-extracted propolis/multiwall carbon nanotubes/gold

Abstract

In this reported work, the electrochemical behaviour of gold electrodes modified with gold nanoparticles, acetone-extracted propolis, multiwall carbon nanotubes and cholesterol oxidase was established for the detection of hydrogen peroxide by using cyclic voltammetry and amperometric techniques. The obtained results confirmed that the current enzymatic biosensor exhibits a fast, highly sensitive, and costeffective detection of cholesterol. Cholesterol in the concentration range of 0.15-0.55 mmol l-1 was determined with a detection limit of 4.9 × 10-5 mol l-1 by the amperometric method, and the sensitivity of the proposed method was found to be 17.38 μA/mmol l-1. Normal electroactive species such as ascorbic acid and glucose in the presence of the constant concentration of cholesterol in the samples do not interfere with the determination. © 2014 The Institution of Engineering and Technology.