Applications of enzymes in the synthesis and hydrolytic study of 2-deoxy-α- D -glucopyranosyl phosphate

Abstract

The labile sugar phosphate 2-deoxy-α-D-glucopyranosyl phosphate has been synthesized enzymically in a two-step process from 2-deoxy-D-glucose-6-phosphate via an intermediate uridine 5′-diphospho-2-deoxy-D-glucose. Rate constants for acid-catalysed (1 M HClO 4 ) hydrolysis at several temperatures were determined by using an enzymic assay to measure remaining substrate. The values obtained were consistent with that anticipated on the basis of known hydrolysis rates for alkyl- and aryl-2-deoxy-D-glucopyranosides.