LC-MS/MS analysis and cytotoxic activity of extract and fractions of Calophyllum soulattri stembark

Abstract

Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cells. The chemical contents of the extract and fractions were analyzed using =liquid chromatography-mass spectrometry (LC-MS/MS). The results showed that methanol extract and n-hexane fractions had a strong cytotoxic activity with IC50 values of 93.6 and 36 µg/mL, respectively. Meanwhile, as the positive control, ethyl acetate and cisplatin fractions have IC50 values of 233 and 16.2 µg/mL, respectively. The LC-MS/MS analysis showed that the extract and fractions contained polyporusterone A, poricoic acid D, polyporusterone F, esculentagenin, and 1-acetyl-3-(methoxy-carbonyl)-β-carboline. Therefore, C. soulattri stem bark extract and fractions have potential activity as an anticancer agent that can inhibit MCF-7 breast cancer cell growth.