Complete genome sequencing of Pandoraea pnomenusa RB38 and Molecular Characterization of Its N-acyl homoserine lactone synthase gene ppnI

7Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.

Abstract

In this study, we sequenced the genome of Pandoraea pnomenusa RB38 using Pacific Biosciences RSII (PacBio) Single Molecule Real Time (SMRT) sequencing technology. A pair of cognate luxI/R homologs was identified where the luxI homolog, ppnI, was found adjacent to a luxR homolog, ppnR1. An additional orphan luxR homolog, ppnR2, was also discovered. Multiple sequence alignment and phylogenetic analysis revealed that ppnI is an N-acyl homoserine lactone (AHL) synthase gene that is distinct fromthose of the nearest phylogenetic neighbor viz. Burkholderia spp. High resolution tandem mass spectrometry (LC-MS/MS) analysis showed that Escherichia coli BL21 harboring ppnI produced a similar AHL profile (N-octanoylhomoserine lactone, C8-HSL) as P. pnomenusa RB38, the wild-type donor strain, confirming that PpnI directed the synthesis of AHL in P. pnomenusa RB38. To our knowledge, this is the first documentation of the luxI/R homologs of the genus Pandoraea.

References Powered by Scopus

MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods

36420Citations
N/AReaders
Get full text

Prokka: Rapid prokaryotic genome annotation

11604Citations
N/AReaders
Get full text

Prodigal: Prokaryotic gene recognition and translation initiation site identification

7561Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Analysis of single nucleic acid molecules in micro- and nano-fluidics

29Citations
N/AReaders
Get full text

Whole-genome sequencing in the prediction of antimicrobial resistance

21Citations
N/AReaders
Get full text

Chania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil

11Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Lim, Y. L., Ee, R., How, K. Y., Lee, S. K., Yong, D., Tee, K. K., … Chan, K. G. (2015). Complete genome sequencing of Pandoraea pnomenusa RB38 and Molecular Characterization of Its N-acyl homoserine lactone synthase gene ppnI. PeerJ, 2015(8). https://doi.org/10.7717/peerj.1225

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 8

57%

Professor / Associate Prof. 3

21%

Researcher 3

21%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 7

44%

Medicine and Dentistry 4

25%

Environmental Science 3

19%

Engineering 2

13%

Save time finding and organizing research with Mendeley

Sign up for free