A framework for immunofluorescence image augmentation and classification based on unsupervised attention mechanism

Abstract

Autoimmune encephalitis (AE) is a common neurological disorder. As a standard method for neuroautoantibody detection, pathologists use tissue matrix assays (TBA) for initial disease screening. In this study, microscopic fluorescence imaging was combined with deep learning to improve AE diagnostic accuracy. Due to the inter-class imbalance of medical data, we propose an innovative generative adversarial network supplemented with attention mechanisms to highlight key regions in images to synthesize high-quality fluorescence images. However, securing annotated medical data is both time-consuming and costly. To circumvent this problem, we employ a self-supervised learning approach that utilizes unlabeled fluorescence data to support downstream classification tasks. To better understand the fluorescence properties in the data, we introduce a multichannel input convolutional neural network that adds additional channels of fluorescence intensity. This study builds an AE immunofluorescence dataset and obtains the classification accuracy of 88.5% using our method, thus confirming the effectiveness of the proposed method.