Expired human blood as an alternative substituent of sheep blood for Streptococcus Sp. growth

Abstract

Using of blood on the agar medium made to be obtained usually uses the sheep's blood, but the sheep's blood is difficult and high cost, so it was taken on the other alternative which could changes that sheep's blood such as the human expire blood. The research aimed to determine affectivity of the expired blood as medium from the Stretpcoccus Sp. growth. The used research method was true experiment by "the post-test only control group design". Packed red sell by NaCl 0,9 % which is centrifugated caused times and added into 100 ml of medium Blood agar Plates (BAP) and Mueller Hinton Agar (MHA) with the difference of concentrate expired blood. Outcome of the research was on the BAP medium, was the higher human expired blood concentration was resulted the narrower, whereas on the MHA medium, was the higher the resistor zone expired bood concentrate was resulted the higher. Conclusion of the research is the optimal concentrate which can is used on the blood agar medium made is 5%-8%.