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High Speed HPLC Analysis of Polyamines in Plant Tissues

  • Walter H
  • Geuns J
Plant Physiology (1987) 83(2) 232-234
DOI: 10.1104/pp.83.2.232
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Abstract

A high speed high performance liquid chromatography (HPLC) method for the quantification of putrescine, spermidine, and spermine in biological samples is described. The dansylation is followed by a sample cleanup. The isocratic HPLC-analysis with acetonitrile:H(2)O (72:28 volume/volume) on 10 centimeter long, 3 micrometer octadecyl silica columns (3 millimeter inner diameter) takes only 4.5 minutes. By our method about 100 analyses can be done in 1 day.

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Walter, H. J.-P., & Geuns, J. M. C. (1987). High Speed HPLC Analysis of Polyamines in Plant Tissues. Plant Physiology, 83(2), 232–234. https://doi.org/10.1104/pp.83.2.232

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