Improvement of glidobactin A production by Polyangium brachysporum

Abstract

Glidobactins A, B and C are lipopeptide antitumor antibiotics produced by the gliding bacterium Polyangium brachysporum sp. nov. No. K481-B101. The production of glidobactin A was examined in shake flasks and laboratory fermentors. Medium screening and optimization led to approximately five fold increases in glidobactin A titers in shake flasks and a ten fold increase in titers in 40-1 batch fermentations. Utilization of a stepped glucose feeding protocol resulted in glidobactin A titers of 1860 μg/ml after 144 h of fermentation. © 1990 Society for Industrial Microbiology.