Reduced Graphene Oxide Based Electrochemical Nonenzymatic Human Serum Glucose Sensor

Abstract

Present work proposes green synthesis of reduced graphene oxide using lemon peel extract(vitamin-c) and its application as an electrochemical nonenzymatic human serum glucose sensor. Improved modified Hummer's method was preferred for the preparation of graphene oxide. X-ray diffraction (XRD), Ultraviolet-visible absorption spectroscopy (UV-Vis) and X-ray photon spectroscopy (XPS) analysis revealed the successful reduction of Graphene oxide (GO) using lemon peel extract. Field emission scanning electron microscopy (FESEM), Fourier transform infra-red spectroscopy (FTIR) and Raman spectroscopy supports the formation of reduced graphene oxide (rGO) nanosheets. The proposed glucose sensor exhibits high sensitivity of 1402 μA cm-2 mM-1 (S/N=3) along with correlation coefficient of 0.9887 and low detection limit of 0.011 μM. The sensor has detected glucose with RSD of 1.99% in human blood serum. The measured values are well agreed with the values obtained using professional glucose sensor used in hospitals.