Synthesis and Crystal Structure of a Phosphorylated Estrogen Receptor Ligand Binding Domain

Abstract

Chemical protein synthesis allows the generation of milligram quantities of correctly folded and previously inaccessible tyrosine phosphorylated estrogen receptor α (ERα) and β (ERβ) ligand binding domains. By using this synthetic strategy, the crystal structure of a post-translationally modified nuclear receptor (pY488 ERβ) could be obtained for the first time (see figure). Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.