Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1

Abstract

Background: Several anti-TNF biologicals are available to treat autoimmune diseases. However, selective TNFR1 inhibition is advisable, thereby reducing the pro-inflammatory TNF/TNFR1 signaling, while the good immunomodulatory TNF/ TNFR2 signaling is preserved. Results: We generated and characterized an anti-TNFR1 Nanobody, TNF Receptor-One Silencer (TROS). Conclusion: TROS inhibits inflammation in vitro, ex vivo, and in vivo. Significance: Anti-TNFR1 therapies are potential novel treatments against autoimmune diseases.