Antifungal Activity and Phytochemical Analysis of Leaves Extract of Medicinal Plant Cassia tora Using GC/MS

Abstract

The extensive evidence for the many pharmacological and therapeutic possibilities may be provided by the several activities of Cassia tora. This study used gas chromatography-mass spectrometry to examine the antifungal activity and phytochemical profile of an extract from the medicinal Cassia tora plant. The resulting crude extracts were further refined with Whatman No. 1 filter paper and kept at 4 °C for future reference. A rotary evaporator operating under vacuum at 40 °C achieved the desired concentration. To determine the chemicals' identities, we used industry-standard techniques to compare their mass spectra to those in the NIST/Wiley internal reference mass spectra library. The antifungal activity of an extract from Cassia tora leaves was tested using the cup plate method, which involves 90% methanol. Dry heat in a hot air oven was used to sterilise petri plates for 1.5 hours at 160 °C. Laminitol, Methyl 1-fluoro-4-oxocyclohexane-1-carboxylate, Hexadecanoic acid, alpha-Linolenic acid, 3,7,11,15-Tetramethylhexadec-1-en-3-ol, alpha-Tocopherol acetate, 6-Dodecanone, 5,8-diethyl-7-hydroxy, Methyl beta-D-glucopyranoside, 1,3-Dioleoyl-2-palmitoylglycerol, 2-propylpentyl 14-methylpentadecanoate, 8-Ethylquinoline-3-carboxylic acid, 1-Allyl-4-methoxy-2,3-dimethylbenzene. Methanol, Ethyl acetate, and Ethanol were Candida albicans, (15.00 ± 0.31, 18.55 ± 0.39 and 21.00 ± 0.43 respectively), Candida glabrata (23.00 ± 0.49, 19.08 ± 0.35 and 26.05 ± 0.51 respectively), Trichophyton rubrum (13.00 ± 0.31, 20.74 ± 0.41 and 24.77 ± 0.50 respectively), Microsporum canis (23.09 ± 0.44, 15.95 ± 0.31 and 17.22 ± 0.36 respectively), Aspergillus niger (10.00 ± 0.27, 09.94 ± 0.18 and 18.45 ± 0.37 respectively), Alternaria Alternaria (11.57 ± 0.21, 08.96 ± 0.17 and 15.90 ± 0.36 respectively), and standard antifungal [Voriconazole (VCZ) and Fluconazole (FCZ)] were (29.00 ± 0.54 and 31.79 ± 0.59) respectively.