Stimulation of neurogenesis at hippocampus in Alzheimer's disease

Abstract

Alzheimer's disease is a chronic neurodegenerative disease, which is characterized by specific pathomorphological changes in brain including hippocampus. One of prospective direction in development of approaches for treatment of Alzheimer's disease is use of gene-cell technologies. In present work we studied the possibility to stimulate neurogenesis at hippocampus of APP/PS1 transgenic mice by transplantation of umbilical cord blood mononuclear cells transduced with adenoviral vector overexpressing recombinant glial neurotrophic factor (GDNF). Effectiveness of therapy was evaluated basing on immunoexpression of stem and progenitor cells (using antibodies against doublecortin and nestin) in different hippocampal areas. Analysis of obtained data showed that transplantation of umbilical cord blood mononuclear cells overexpressing glial neurotrophic factor or enhanced green fluorescent protein stimulates processes of neurogenesis at hippocampus of transgenic mice with Alzheimer's disease model, certifying high therapeutic potential of these gene-cell constructs.