Intensification of Host's Immunity by Squalene in Sarcoma 180 Bearing Icr Mice

Abstract

Effect of highly purified squalene (HP-SQ) obtained from liver of a shark, Centro-phorus atromarginative GARMAN, on antitumor activity and host's immune response was studied in sarcoma 180 bearing female ICR mice. HP-SQ was administered intraperitoneally (i.p.) to solid tumor-bearing or healthy mice at the dose of 0.1 ml/mouse/d for consecutive 10 d. The administration or HP-SQ exerted the host's resistance against challenging 5 X104 cells of ascites sarcoma 180, and it resulted in marked prolongation of survival of the mice (ILS; 110%). Function of the reticuloendothelial system estimated by a carbon clearance test was enhanced by i.p. administration of HP-SQ in both the healthy and the tumor-bearing mice. Number of IgM antibody forming cells against sheep red blood cells (SRBC) in the spleen was counted 4 d after intravenous injection of 2 x IQ7 cells of SRBC. The number of the IgM antibody forming cell increased by HP-SQ administration in the tumor-bearing and the healthy mice. Delayed hypersensitivity reaction against SRBC measuring swelling of the foot pads of mice was also stimulated by the i.p. administration of the material. © 1983, The Pharmaceutical Society of Japan. All rights reserved.