Preparation of platelet-rich plasma as a tissue adhesive for experimental transplantation in rabbits

Abstract

Purpose: Platelet-rich plasma (PRP) is an autologous substance with adhesive properties. We aimed at developing and testing the efficacy of a method for PRP preparation in rabbits. Materials and methods: An in vitro study was carried out to obtain PRP from forty rabbits and to ahalyze the number of platelets and type of substance needed to trigger platelet activation. To induce platelet activation, 5%, 10%, 25% and 50% CaCl solutions were used. Then, an in vivo study was performed in twelve rabbits to test PRP adhesiveness in lamellar corneal graft. A control group made up of six rabbits underwent corneal transplantation without using PRP. Results: 5% CaCl was the most effective concentration in activating PRP, with a mean time of 19 minutes. An attached comeal flap was seen 3 months after surgery. A detached corneal button was seen in all controls. Conclusion: Our method was able to produce rabbit-derived PRP with suitable properties for soft tissue adhesion. These results could be useful for researchers of the growing fields of tissue repair and experimental transplantation. © 2006 Luengo Gimeno et al; licensee BioMed Central Ltd.