Isolation and Diagnosis of Resistant Bacteria from Pregnant Women with Urinary Tract Infections in Karbala

Abstract

Background & Objective: Increasing urinary tract infections (UTI) in pregnant women was a reason for which this study aimed to find out the spread of bacteria in them and also study the resistance of bacteria to antibiotics and the type of resistance. Materials & Methods: During November 2022, 50 urine samples were collected from pregnant women with symptoms of UTI. Direct microscopy tests were conducted on the samples. The samples were cultured on the media of MacConkey agar (MAC) and blood agar. Biochemical tests were performed and diagnosed using the VITEC-2 system. Antibiotic susceptibility screening test was done for all isolates. Results: Of the 50 bacterial isolates diagnosed and isolated from pregnant women with UTI, 84% were gram-negative and 16% were gram-positive. The most prevalent bacteria were E. coli, with a rate of 60%, followed by Proteus mirabilis, with a rate of 12%. All E. coli isolates were resistant (100%) to the AMOX antibiotic, and the isolates showed high resistance (87%) to CFR, CN, CZ, CXM, CAE, CPD, CRO NA and SXT antibiotics. 13.3% of E.coli isolates were extended detection and response (XDR), 50% and 25% of Staphylococcus hominis and aureus isolates were XRD, respectively. Conclusion: E. coli is the most common and most resistant bacteria of type XRD, and gram-positive bacteria, staph bacteria, showed resistance to type XRD. In addition, gram-negative bacteria showed high resistance to many antibiotics, including AMOX, CFR, CN, CZ, CXM, and CAE. Gram-positive bacteria showed complete resistance against BENPEN, OXA, CLIN, TEC, VAN, TET, FUS and VAN.