Effect of 2-methoxyethanol induction on mice (Mus musculus) liver, kidney and ovary

Abstract

These research was design to evaluate the effect of 2-methoxyethanol (2-ME) injection to the number of damaged hepatocyte, renal tubule, and follicle. This study was designed as Completely Randomized Design experimental study. Animal model used was 2-3 months old 20 female mice strain Balb/C mice weighed at 25-30 g. Mice were divided into 4 treatment groups; N (normal control), G1 (given 200 mg/kg BW 2-ME), G2 (given 250 mg/kg BW 2-ME), and G3 (given 300 mg/kg BW 2-ME). 2-ME was administered intraperitoneally daily for 5 consecutive days. Liver, kidney, and ovarium were removed at the end of treatment period then processed into histological slides. Damage on hepatocyte, nephron, and follicle was evaluated from tissue sections. Data collected was analyzed statistically. Result showed that level of damage increased along with 2-ME dose given to mice. KP3 was found to have highest number of damaged cell in the three organs, followed by KP2, and KP1 had the lowest number of damaged cells compared to KN. Injection of 2-ME at various doses could induce damage on hepatocyte, renal tubule, and ovarian follicle.