Comparative Plastomes and Phylogenetic Analysis of Cleistogenes and Closely Related Genera (Poaceae)

7Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Cleistogenes (Orininae, Cynodonteae, Chloridoideae, Poaceae) is an ecologically important genus. The phylogenetic placement of Cleistogenes and phylogenetic relationships among Cleistogenes taxa remain controversial for a long time. To resolve the intra- and inter-generic relationships of Cleistogenes, the plastomes of 12 Cleistogenes taxa (including 8 species and 4 varieties), one Orinus species, 15 Triodia species, two Tripogon species, and two Aeluropus species were included in the present study. All the taxa showed a similar pattern in plastome structure, gene order, gene content, and IR boundaries. The number of simple sequence repeats ranged from 145 (O. kokonorica) to 161 (T. plurinervata and T. schinzii). Moreover, 1,687 repeats were identified in these taxa, including 1,012 forward, 650 palindromic, 24 reverse, and one complement. Codon usage analysis revealed that these plastomes contained 16,633 (T. stipoides) to 16,678 (T. tomentosa) codons. Sequence divergence analysis among Cleistogenes and closely related genera identified five non-coding regions (trnS-UGA-psbZ, rpl32-trnL-UAG, trnQ-UUG-psbK, trnD-GUC-psbM, trnT-GGU-trnE-UUC). Phylogenetic analysis of complete plastomes indicated that Cleistogenes is sister to a clade composed of Orinus and Triodia, whereas it did not support the sister relationship between the recently proposed subtribe Orininae (Cleistogenes and Orinus) and Triodia. The subtribe Orininae was not supported by our complete plastome data. The split between Cleistogenes and Orinus-Triodia clade go back to 14.01 Ma. Besides, our findings suggested that C. squarrosa and C. songorica are the successive early diverging groups in the phylogenetic analysis. The other 10 taxa are divided into two groups: a monophyletic group composed of Cleistogenes sp. nov. and C. caespitosa var. ramosa is sister to other eight Cleistogenes taxa. Cleistogenes was estimated to have experienced rapid divergence within a short period, which could be a major obstacle in resolving phylogenetic relationships within Cleistogenes. Collectively, our results provided valuable insights into the phylogenetic study of grass species.

References Powered by Scopus

MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets

37313Citations
N/AReaders
Get full text

MAFFT multiple sequence alignment software version 7: Improvements in performance and usability

31106Citations
N/AReaders
Get full text

RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies

25017Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Comparative and Phylogenetic Analysis of Complete Plastomes among Aristidoideae Species (Poaceae)

9Citations
N/AReaders
Get full text

The newly assembled chloroplast genome of Aeluropus littoralis: molecular feature characterization and phylogenetic analysis with related species

3Citations
N/AReaders
Get full text

Plastid Phylogenomics of Paeonia and the Evolution of Ten Flower Types in Tree Peony

2Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Wang, R., Liu, K., Zhang, X. J., Chen, W. L., Qu, X. J., & Fan, S. J. (2021). Comparative Plastomes and Phylogenetic Analysis of Cleistogenes and Closely Related Genera (Poaceae). Frontiers in Plant Science, 12. https://doi.org/10.3389/fpls.2021.638597

Readers over time

‘21‘23‘2400.751.52.253

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 4

80%

Professor / Associate Prof. 1

20%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 3

75%

Biochemistry, Genetics and Molecular Bi... 1

25%

Article Metrics

Tooltip
Mentions
References: 1

Save time finding and organizing research with Mendeley

Sign up for free
0