Molecular and kinetic characterization of lactate dehydrogenase enzyme in the heart and breast muscle of Japanese quail (Coturnix japonica)

Abstract

In the present investigation, attempts were made to characterize lactate dehydrogenase (LDH) isozyme in quail heart and breast muscle by using electrophoresis, kinetic, and molecular techniques. Polyacrylamide gel electrophoresis of the heart and breast muscle revealed a single isozyme. Kinetic studies were carried out for further functional characterization of LDH isozyme in the heart and breast muscle. Substrate inhibition, enzyme kinetics, and inhibitor (urea) studies indicated that the isozymes present in these tissues diff ered in their functional properties. LDH isozyme present in the heart and breast muscle exhibited properties of LDH-A and LDH-B isozyme, respectively. Furthermore, RT-PCR amplifi cation of Ldh-a and Ldh-b mRNA in both tissues confi rmed that these isozymes were formed by the association of both H and M subunits and may be of H3M1 and M3H1 in the heart and breast muscle, respectively. © TÜBİTAK.