The regulatory role of fos related antigen-1 in inflammatory bowel disease

Abstract

The etiology of inflammatory bowel disease (IBD) remains unclear. The ratio of Fos related antigen-1 (Fra-l)-positive intestinal mucosa epithelial cells is significantly increased in active IBD. This study intends to explore the regulatory role of Fra-1 in IBD. The Fra-1 eukaryotic expression vector was constructed and stably transfected to establish the Fra-1 overexpression HCT-116 (116-Fra-1) intestinal epithelial cell line. The impact of Fra-1 overexpression on intestinal mucosal epithelial cell damage repair function was tested using a scratch assay. The role of Fra-1 overexpression on intestinal mucosal epithelial cell proliferation was evaluated using a Cell Counting Kit-8 assay. Apoptosis related proteins, B-cell lymphoma 2 (Bcl-2), c-Myc, Survivin and Bcl-extra large (Bcl-xL), expression levels were detected by western blotting. Fra-1 suppressed intestinal mucosal epithelial cell damage repair and proliferation. Fra-1 inhibited the protein levels of Bcl-2, c-Myc, Survivin, and Bcl-xL. Fra-1 overexpression in intestinal mucosal epithelial cells may restrain damage repair after intestinal mucosal injury in IBD remittent period through weakening the protective effect of intestinal mucosa, thus increasing the risk of recurrence. Therefore, suppressing Fra-1 expression in intestinal mucosal epithelial cells may contribute to IBD remittent maintenance and recurrence delay.