Application of microsatellite markers as marker assisted selection (mas) in the f3 generation results crosses of black rice and white rice

Abstract

The study aims to assess the use of microsatellite markers as DNA marker assisted selection in F3 generation of the cross between black rice with white rice. The research was conducted in the fields and the laboratory of Genetics and Plant Breeding of UGM Yogyakarta. The research material consisted plant populations ofF3 S x G, black rice/S, Situbagendit white rice/G, chemicals and tools for the activity of molecular, and RM 252 microsatellite markers. Population of F3 generation S x G, black rice as female parents (S), and white rice (G) as a male parent were grown in fields to harvesting. Young leaves all plant population (S, G, F3 S x G) were analyzed of molecularly using the RM 252 microsatellite markers. The results showed that the RM 252 microsatellite markers effectively used as a DNA marker-assisted selection tool on the result of black rice with white rice cross breeding.