Evaluation of protective and therapeutic role of zinc oxide nanoparticles and aloin on dextran sulfate-induced ulcerative colitis in rats

Abstract

This study designed to investigate the anti-inflammatory effect of zinc oxide nanoparticles (ZnONPs) and \ or aloe component (aloin) on inflammatory mediators and oxidative stress in dextran sulfate sodium salt induced ulcerative colitis(UC) in rats. sixty-four albino rats divided into eight equal of eight rats each. Group 1:(normal control) received no drugs , group 2:(ulcerative colitis) rats received dextran sulfate sodium salt 3% in drinking water, group 3:(ZnONPs) rats administered ZnONPs (5mg/kg body weight) orally for 3weeks, group 4: (aloin) rats administered orally with 1ml of aloin 0.1% daily for 3weeks, group 5: (aloin + ZnONPs) rats orally administered with NZnO (5mg/kg body weight) and (aloin 0.1%), daily for 3weeks, group 6: (ZnONPs +UC) rats with ulcerative colitis orally treated with ZnONPs (5mg/kg body weight) daily for 3 weeks, group7: (aloin+UC) rats with ulcerative colitis orally treated with (aloin 0.1%), daily for 3weeks, group 8: (aloin+ ZnONPs +UC) rats with ulcerative colitis orally treated daily with ZnONPs (5mg/kg body weight)+aloin (1ml aloin 0.1%) for 3weeks. The obtained results revealed that, administration of ZnONPs and\or aloin to rats with ulcerative colitis significantly reduced elevated serum total cholesterol and TG concentrations, and markedly increased the reduced HDL-C level. On the other hand, elevated level of COX-2, IL-6, MDA and TNF-α in UC rats were significantly reduced, with significant increase of the reduced level of GSH. Results suggest that ZnONPs modulates UC, while aloin showed high efficacy to normalize UC tissues and may considered as potential treatment for UC and other inflammatory bowel disease.