Molecular profiling and genetic diversity of mecA-mediated methicillin-resistant staphylococcus aureus isolates from healthcare workers in a major hospital

Abstract

Objectives: To investigate the genetic diversity and molecular characteristics of methicillin-resistant staphylococcus aureus (MRSA) isolates from healthcare workers in a Jordanian hospital, focusing on the role of the mecA gene in antibiotic resistance. Methods: A total of 25 staphylococcus aureus isolates were collected from healthcare workers’ skin, nasal passages, and environmental sources. Biochemical tests identified MRSA isolates, and DNA extraction was followed by polymerase chain reaction amplification of the 16SrRNA gene for genetic analysis. The sequences were analyzed using phylogenetic methods, sequence demarcation tool (SDT) analysis, and median-joining network analysis to determine genetic diversity and evolutionary relationships. Results: Out of the 25 staphylococcus aureus isolates, 10 were identified as methicillin-resistant. Phylogenetic analysis revealed distinct clustering patterns, indicating genetic heterogeneity. SDT analysis highlighted the genetic complexity of the MRSA strains. The median-joining network identified haplotype 1 as the predominant haplotype, underscoring significant genetic diversity. The mecA gene was detected in all MRSA isolates, emphasizing its critical role in methicillin resistance. Conclusion: The study demonstrates significant genetic diversity among MRSA isolates from healthcare workers in a Jordanian hospital, with the mecA gene playing a vital role in antibiotic resistance. These findings underscore the importance of molecular diagnostics in MRSA detection and the need for targeted infection control strategies to manage antibiotic resistance effectively in healthcare settings.