Substrate inhibition in lipase-catalysed transesterification of mandelic acid with vinyl acetate

Abstract

Kinetic resolution of (R)- and (S)-mandelic acid by its transesterification with vinyl acetate catalysed by Burholderia cepacia lipase has been studied The influence of the initial substrate concentration on the kinetics of process has been investigated A modified ping-pong bi-bi model of enzymatic transesterification of (S)-mandelic acid including substrate inhibition has been developed The values of kinetic parameters of the model have been estimated We have shown that the inhibition effect revealed over a certain threshold limit value of the initial concentration of substrate.