Effect of additives on enhanced in-vitro shoot multiplication and their functional group identification of Chlorophytum borivilianum Sant. Et Fernand

Abstract

The present study was commenced with the aim to optimize the proficient in vitro regeneration protocol and phytochemical constituents and their functional groups identification through FT-IR spectrum analysis. As per of our knowledge, very few or no reports are available on the effects of additives on the nodal explant of C. borivilianum. Surface sterilized explants viz. nodal segments were inoculated on MS basal medium supplemented with 19 different concentrations of cytokinins (BAP, Kinetin and zeatin). BAP at a concentration of 2 mg ml−1 was found superior out of three cytokinins for shoot induction. This study confirmed that the inclusion of additives (Adenine sulphate, casein hydrolysate and putrescine) had a promoting effect on multiple shoot induction and shoot proliferation. Best response was achieved on MS medium supplemented with 2.0 mg l−1 BAP + 1.0 mg l−1 NAA + 20.0 mg l−1 AdSO4 from nodal explant on 45th day of culture. For in vitro rooting, the semi solid rooting medium with IBA (2.0 mg l−1) + putrescine (50.0 mg l−1) was found to be best with 90% maximum field survival rate. The proposed protocol can be used for the conservation of germplasm and propagation of these endangered plant species. FT-IR spectroscopic analysis revealed the presence of alcohols, phenols, alkanes, alpha and beta-unsaturated aldehydes, ketones, aromatic amines, alkyl halides, aliphatic amines, ether linkage and alkenes might be responsible for the various medicinal properties of test plant.