Seed Priming with PEG 6000 and Silicic Acid Enhances Drought Tolerance in Cowpea by Modulating Physiological Responses

Abstract

Cowpea is a nutritionally and economically valuable legume, known for its adaptability to adverse conditions. However, water stress negatively affects its development, requiring technologies to enhance resilience. This study aimed to induce tolerance to water deficit in cowpea through seed priming with polyethylene glycol 6000 (PEG 6000) and silicic acid. A completely randomized experiment was conducted in a phytotron chamber with two water regimes (W50 and W100) and six seed priming treatments, with four replications. Priming consisted of three water potentials induced by PEG 6000 (0 MPa, −0.4 MPa, and −0.8 MPa) and two silicon concentrations (0 and 200 mg L−1). Gas exchange parameters, including photosynthetic rate (A), transpiration rate (E), stomatal conductance (gs), intercellular CO2 concentration (Ci), instantaneous water use efficiency (WUEi), and instantaneous carboxylation efficiency (iCE), were evaluated. Seed priming with PEG 6000 and silicon improved A, WUEi, and iCE under water deficit. Treatments 2 (0 MPa + 200 mg L−1 Si), 3 (−0.4 MPa + 0 mg L−1 Si), and 4 (−0.4 MPa + 200 mg L−1 Si) enhanced gas exchange, suggesting an effective strategy for improving drought tolerance in cowpea and ensuring food security.