An improvised machine learning model based on mutual information feature selection approach for microbes classification

Abstract

The accurate classification of microbes is critical in today’s context for monitoring the ecological balance of a habitat. Hence, in this research work, a novel method to automate the process of identifying microorganisms has been implemented. To extract the bodies of microorganisms ac-curately, a generalized segmentation mechanism which consists of a combination of convolution filter (Kirsch) and a variance-based pixel clustering algorithm (Otsu) is proposed. With exhaustive corroboration, a set of twenty-five features were identified to map the characteristics and morphol-ogy for all kinds of microbes. Multiple techniques for feature selection were tested and it was found that mutual information (MI)-based models gave the best performance. Exhaustive hyperparameter tuning of multilayer layer perceptron (MLP), k-nearest neighbors (KNN), quadratic discriminant analysis (QDA), logistic regression (LR), and support vector machine (SVM) was done. It was found that SVM radial required further improvisation to attain a maximum possible level of accuracy. Comparative analysis between SVM and improvised SVM (ISVM) through a 10-fold cross validation method ultimately showed that ISVM resulted in a 2% higher performance in terms of accuracy (98.2%), precision (98.2%), recall (98.1%), and F1 score (98.1%).