Glucosylglycerol on performance of prebiotic potential

Abstract

Background: Glucosylglycerol (GG), an organic compound constitutes a structurally diverse group of small organic molecules, which has a glycosidic chemical structure, a powerful osmolyte which is catalyzed by sucrose phosphorylase in the presence of sucrose and glycerol as a donor and acceptor substrate. Context and purpose of this study: This research aims to study the potential of prebiotic properties from GG compared with three commercial oligosaccharides (FOS, inulin and lactulose) which also have prebiotic properties. Results: The cultures with GG and FOS showed statistically significant difference (P<0.05) capacity to stimulated both probiotic strains (Lactobacillus acidophilus and Lactobacillus plantarum) and presented high ability to inhibit four intestinal pathogens (Bacillus cereus, Escherichia coli, Samonella paratyphi and Staphyloccous aureus), as displayed from wider inhibition zone compared with the culture without oligosaccharide. The tolerance ability of different gastrointestinal conditions (alpha-amylase, bile extract and HCl) in the culture medium with GG presented the highest percentage of survival (6.50%) after being incubated with HCl for 3 hours (significant difference as P<0.05). The culture of L. acidophilus with GG in the medium displayed maximum lactic acid concentration (1.46 mg/mL) after 48 incubation hours. Conclusions: GG has high potential of prebiotic properties for probiotic growth stimulation, pathogenic inhibition and gastrointestinal tolerance. Additionally, GG can introduce probiotic for high production of lactic acid concentration in the culture media. From these results, GG is promising for application and development to become an effective functional food in the coming future.