Protective impacts of Cupressus sempervirens leaves extracts against paracetamol hepatotoxicity

Abstract

The main objective of this study is to evaluate the antioxidant activity of Cupressus sempervirens leaves extract invitro and rat model in which paracetamol was used to induce hepatic damage. The antioxidant and anti-quorum sensing activities of eight extracts were studied. Total phenolic compounds and scavenging radical effect on 2, 2-diphenylpicrylhydrazyl (DPPH) were investigated, (the specific phenolic and flavonoid composition quantification for hydrolyzed ethyl acetate fraction pH 4 were performed by HPLC E-vanilic, Alpha-coumaric, Salicylic and Ferulic were found as the major phenols. Narengin, Hisperidin, Quercetrin and Hispertin were found as the major flavonoids. Biological experiment was carried out for thirty days. Results revealed that a significant increase in serum ALT, AST, ALP, Total bilirubin, TG, Total cholesterol, (LDLC) and nitric oxide in rats treated with paracetamol. However administration of Cupressus sempervirens in paracetamol induced liver toxicity in rats exhibited a significant decrease in all mentioned parameters on the other hand a significant decrease in serum total protein albumin concentration, (HDLC), Catalase, glutathione peroxidase (GPX), Glutathione-S-transferase (GST) and Glutathione reduced were observed in paracetamol induced hepatic toxicity in rats when compared with control normal group. Meanwhile Cupressus sempervirens leaves resulted in significant in all mentioned parameter and when enhanced the activity of antioxidant enzymes in liver tissue. It could be concluded that, inhibition of peroxidation, inflammation and oxidative stress and enhanced antioxidant status in rat liver tissue by Cupressus sempervirens suggest the potential efficacy of Cupressus sempervirens as an addition Hepatoprotective, anti-inflammatory and anti-hepatotoxic agent in treatment of liver toxicity