Loop-mediated isothermal amplification for influenza A (H5N1) virus

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Abstract

We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.

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Jayawardena, S., Cheung, C. Y., Barr, I., Chan, K. H., Chen, H., Guan, Y., … Poon, L. L. M. (2007). Loop-mediated isothermal amplification for influenza A (H5N1) virus. Emerging Infectious Diseases, 13(6), 899–901. https://doi.org/10.3201/eid1306.061572

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