Application of Aglao E Unhas Compound from Aglaophenia cupressina Lamoureoux against Colletotrichum gloesporioides on Strawberry and Aspergillus niger on Mango

Abstract

This study aimed to extract and characterize the main bioactive compound of Aglaophenia cupressina Lamoureoux using UV, IR, and NMR spectroscopy. This compound was then tested in vitro using the agar diffusion method at a concentration of 15, 30, and 45 ppm against two fungi isolated from the spoiled strawberry and mango. The efficacy of Aglao E Unhas was tested in fully ripe strawberries and mangoes. The number of fungi that grew during storage was counted using the standard plate count method. The results of the isolation of hydroid A.cupressina yielded the pure compound Aglao E Unhas derivative of alkaloids, which is a new compound, in the form of white crystals, melting point at 55–56 ˚C, which has 15 carbon atoms and 39 hydrogen atoms, one NH group in a heterocyclic ring. Aglao E Unhas at 45 ppm has antifungal properties against Colletotrichum gloesoporioides and Aspergillus niger. The largest inhibition zone against Colletotrichum gloesoporioides was 18.20 mm at 48 h and increased to 18.70 mm at 72 h of incubation. The inhibition zone against Aspergillus niger at 48 hours of incubation resulted in 17.35 mm and 72 h of incubation increased to 18.00 mm.