Development of online solid-phase extraction-HPLC/MS/MS method for the determination of perfluorochemicals in human plasma

Abstract

A method for determining perfluorochemicals (PFCs) such as perfluorooctanesulfonic acid (PFOS), paerfluorooctane slufoneamide (PFOSA), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA) and perfluorodecanoic acid (PFDA), in human plasma samples was developed by online solid-phase extraction-HPLC/MS/MS, only after deproteination with acetonitrile. The limits of detection of PFOS, PFOSA, PFOA, PFNA and PFDA in human plasma at a signal to noise (ratio of 3) were 0.08-0.14 ng/ml, and the limits of quantitation of PFOS, PFOSA, PFOA, PFNA and PFDA in human plasma were 0.50 ng/ml. The average recoveries of PFOS, PFOSA, PFOA, PFNA and PFDA ranged from 93.3 to 105% (RSD, 3.0-8.9%; n = 6). This method is more rapid and accurate, compared with the column-switching HPLC/MS method presented in previous reports 18)19). The developed method can be applied to the determination of PFOS, PFOSA, PFOA, PFNA and PFDA in human plasma samples for monitoring human exposure. © 2005 The Japan Society for Analytical Chemistry.