Increased profile fertilization and the process of cleavage of kacang goat in vitro fertilization using intracytoplasmic sperm injection

Abstract

The weakness of in vitro fertilization (IVF) was not able to observe whether in vitro fertilized oocytes were all mature. Intra Citoplasmic Sperm Injection (ICSI) was able to solve IVF failure as spermatozoa was directly injected to oocyte. The research was divided into 2 groups, T1 group: oocyte was fertilized conventionally (maturated oocyte was supplamanted with spermatozoa) and T2 group: fertilization using ICSI method. Next, oocyte was mixed with spermatozoa. After ICSI was done, it was put into incubator CO2 5 %, with the temperature of 38.5oC. Then, fertilization rate and embryo growth at cleavage stage were observed. The data presented as means Standard Error of the Mean (S.E. M) and were compared using the Student's t-test. Research result showed that fertilization rate of ICSI group was higher significant than conventional IVF group (p<0.05). Also, cleavage stage (2 cells, 4 cells, 8 cells) of ICSI group was higher significant than conventional IVF group (p<0.05). The research concluded that fertilization rate and embryo cleavage of IVF using ICSI technique were higher than conventional IVF. Copyright