Method for purification of fluorescence-labeled oligosaccharides by pyridylamination

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Abstract

We developed a convenient method for purification of PA-oligosaccharides to remove contaminants originating from natural fluorescent materials, and excess reagents as well as by-products of tagging reactions in glycan analysis. The method, using a C18-cartridge, is simple and powerful to remove them. Several examples of experiments that showed the usefulness of this purification method are described in this report. © 2002, Taylor & Francis Group, LLC. All rights reserved.

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Natsuka, S., Adachi, J., Kawaguchi, M., Ichikawa, A., & Ikura, K. (2002). Method for purification of fluorescence-labeled oligosaccharides by pyridylamination. Bioscience, Biotechnology and Biochemistry, 66(5), 1174–1175. https://doi.org/10.1271/bbb.66.1174

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