Study on the properties of halomonas urease and optimization of its production conditions

Abstract

It is of great significance to treat the wastewater of high salt urea by using the halophilic microorganism containing urease. In this paper, Halomonas containing urease was selected to study its urease properties and optimize its urease production conditions. Halomonas sp. H36 was selected, and its growth reached its peak at 36 h. The maximum value was 7.42, and the urease activity was 63.38 U. In the study on urease properties of this strain, when pH = 8, temperature was 50℃, salt concentration was 60 g/L, and urea concentration was 50 g/L, urease activity reached 137.52 U, increasing by 116.98%. It was found that the urease activity remained at 73.53% when the salt concentration reached 150 g/L. It was found by response surface method that glucose, ammonium sulfate and sodium chloride were the key factors affecting urease activity of the strain. After response surface optimization, the urease activity reached 93.628 U, increasing by 47.72%. This is of great significance to the wastewater treatment of high-salt urea and has a broad application prospect in the sewage treatment of ships.