Short communication. efficiency of AFLP markers and seed storage protein electrophoresis to study the phylogeny of some Hordeum species

Abstract

This study is focused on reconstructing the phylogeny of 60 accessions representing ten species of the genus Hordeum based on amplified fragment length polymorphism (AFLP) markers and SDS-PAGE of seed storage proteins. We obtained 339 polymorphic AFLP bands and 46 polymorphic protein bands from the SDS-PAGE of water soluble and non-soluble seed storage proteins. The phylogenetic tree deduced from AFLP analysis is concordant with that deduced from seed storage proteins electrophoresis. The studied taxa were clustered according to their genome type into two main groups representing the Old and New World's species. Inside each group the species were clustered according to their genome type. Highly significant cophenetic correlation coefficients obtained in both AFLPs (0.96) and seed storage proteins (0.89) indicate the reliability of the results. It can be concluded that both AFLP and SDS-PAGE are adequate techniques to study the Hordeum phylogeny.