Senescence associated β galactosidase activity in human retinal pigment epithelial cells exposed to mildhyperoxia in vitro

Abstract

Aims: To determine if mild hyperoxia induces senescence in retinal pigment epithelium (RPE) cells in vitro. Methods: RPE340 cells and W138 cells were grawn in 20% oxygen and 40% oxygen until proliferative exhaustion. A combined senescence associated β galactosidase (SABG) and 5-bromo-2′-deoxyuridine (BrdU) double labelling technique was performed at different times and labelled cells were counted. Results: Cells grown in 40% oxygen stopped proliferating at an earlier population doubling level than when grown in 20% oxygen. An increase in SABG positive cells and decrease of BrdU positive cells in 40% oxygen developed at an earlier time than when grown in 20% oxygen. Conclusion: Mild hyperoxia induces premature senescence in a specific RPE cell line.