In-vitro antimicrobial activity of ethyl acetate extract of two common edible mushrooms

Abstract

Ethyl acetate extracts of the reishi (Ganoderma lucidum) and oyster (Pleurotus ostreatus) mushrooms were tested for their in vitro growth inhibitory activity against a panel of microorganisms of reference strains. Antimicrobial effects of both mushrooms were monitored in the dose of 400 micro gm /disc by disc diffusion method using five Gram-positive bacteria, five Gram-negative bacteria and three fungi as well. Present study showed that ethyl acetate extract of Pleurotus ostreatus has moderate and Ganoderma lucidum has only mild antibacterial effect in comparison with standard Kanamycin (30 gm. /disc). However, both mushrooms had no antifungal activity on the experimental fungi compared with standard drug Nystatin at the dose of 30 gm. /disc.