Cell Therapy and Investigation of the Angiogenesis of Fibroblasts with Collagen Hydrogel on the Healing of Diabetic Wounds

Abstract

Objectives: A diabetic ulcer is a common disease in patients with diabetes. Because of antibiotic resistance, new therapeutic alternatives are being considered in diabetic foot patients to reduce complications and mortality. This study aimed to evaluate the effect of collagen hydrogel on the wound-healing process in diabetic rats. Materials and Methods: Diabetic wounds were induced with streptozotocin in all 42 male Wistar rats. The rats were divided into four groups: (a) treated with fibroblast cells, (b) collagen hydrogel, (c) collagen cultured with fibroblast cells, and (d) a control group. Microscopic and histological (hematoxylin and eosin staining and Mason trichrome staining), measurement of wound surface with image J, skin density and thickness by the ultrasound probe, and skin elasticity with cytometer tool were used to evaluate wound healing at days 14 and 21 after the treatment. Results: The results showed that treating diabetic wounds with fibroblasts cultured in collagen hydrogel greatly reduces inflammatory responses in the skin tissue and significantly accelerates the healing process. In addition, 21 days after the start of treatment, skin elasticity, thickness, and density were higher in the collagen + fibroblast group than in the control group. Conclusion: In addition, the results of the present study show that diabetic wound dressing can significantly reduce the inflammatory phase in the wound healing process by increasing the speed of collagen synthesis, skin density and elasticity, and angiogenesis.