Degradation of [ Dha 7 ]MC-LR by a Microcystin Degrading Bacterium Isolated from Lake Rotoiti, New Zealand

  • Somdee T
  • Thunders M
  • Ruck J
  • et al.
N/ACitations
Citations of this article
29Readers
Mendeley users who have this article in their library.

Abstract

For the first time a microcystin-degrading bacterium (NV-3 isolate) has been isolated and characterized from a NZ lake. Cyanobacterial blooms in New Zealand (NZ) waters contain microcystin (MC) hepatotoxins at concentrations which are a risk to animal and human health. Degradation of MCs by naturally occurring bacteria is an attractive bioremediation option for removing MCs from drinking and recreational water sources. The NV-3 isolate was identified by 16S rRNA sequence analysis and found to have 100% nucleotide sequence homology with the Sphingomonas MC-degrading bacterial strain MD-1 from Japan. The NV-3 isolate (concentration of 1.0 × 10 8 CFU/mL) at 30°C degraded a mixture of [Dha 7 ]MC-LR and MC-LR (concentration 25 μ g/mL) at a maximum rate of 8.33 μ g/mL/day. The intermediate by-products of [Dha 7 ]MC-LR degradation were detected and similar to MC-LR degradation by-products. The presence of three genes ( mlr A, mlr B, and mlr C), that encode three enzymes involved in the degradation of MC-LR, were identified in the NV-3 isolate. This study confirmed that degradation of [Dha 7 ]MC-LR by the Sphingomonas isolate NV-3 occurred by a similar mechanism previously described for MC-LR by Sphingomonas strain MJ-PV (ACM-3962). This has important implications for potential bioremediation of toxic blooms containing a variety of MCs in NZ waters.

Cite

CITATION STYLE

APA

Somdee, T., Thunders, M., Ruck, J., Lys, I., Allison, M., & Page, R. (2013). Degradation of [    Dha  7    ]MC-LR by a Microcystin Degrading Bacterium Isolated from Lake Rotoiti, New Zealand . ISRN Microbiology, 2013, 1–8. https://doi.org/10.1155/2013/596429

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free