Isolation and Characterisation of Flavonoids (Rutin) from the Roots of Cadaba aphylla (Thunb) and Adenia glauca of Potential in Anti-Oxidant activity

Abstract

Natural medicine is widely used to treat a variety of ailments. This work aims to isolate and characterise flavonoids from the roots of Cadaba aphylla and Adenia glauca. The compound was extracted using the Ultrasonicate Assisted Extraction (UAE), and Chromatographic methods including thin layer chromatography (TLC) and column chromatography were then used to separate it. The isolated compound was then examined using UV-visible, FTIR, NMR (H1 & C13), and mass spectra (MS) followed by antioxidant evaluation. From the qualitative and spectral examination. The isolated component is identified as 3, 3', 4', 5, 7-pentahydroxy flavones-3-rutinoside, it is concluded (Rutin) and it also showed good free radical scavenging behaviour. Therefore, these plants can be used for therapeutic purpose.